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Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey BABT
Gomaa,Eman Zakaria.
The aim of the present study was to investigate the antimicrobial effect of the lipopeptide biosurfactants produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using the disc-diffusion method against different Gram-positive bacteria {B subtilis, B. thuringiensis (two strains), B. cereus, Staphylococcus aureus (two strains) and Listeria monocytogenes)}, Gram-negative bacteria {(Pseudomonas aeruginosa, Escherichia coli (two strains), Salmonella typhimurium, Proteous vulgaris and Klebsiella pneumoniae) and a yeast (Candida albicans)}. The biosurfactant showed profoundly distinct antibacterial activity toward tested bacteria and displayed an antifungal activity against the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Bacillus licheniformis; Cheese whey; Antimicrobial activity; Staphylococcus aureus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200011
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Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes BJM
Gomaa,Eman Zakaria.
The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of 72 h. The best nitrogen sources for protease and CMCase production were yeast extract and casein, respectively. Also maximum protease and CMCase production were reported at pH 9 and pH 10, respectively. The enzymes possessed a good stability over a pH range of 8-10, expressed their maximum activities at pH10 and temperature range of 30-50 °C, expressed their maximum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaliphillic; Protease; Carboxymethylcellulase; Production; Characterization.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200033
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Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol BABT
Gomaa,Eman Zakaria.
The aim of this work was to study the production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli isolated from the industrial contaminated soil samples using cane molasses as an inexpensive substrate. The amount of PHA accumulated followed a similar pattern to its growth for each of treatment indicating a growth-related production, yielding maximum PHA production of 54.1 and 47.16% for B. subtilis and E. coli, respectively after 96 h cultivation in the medium containing 6 and 8 % molasses, respectively and decreased thereafter. The growth and PHA yields were improved by introducing 1% ethanol into the molasses medium. Ammonium sulphate and ammonium nitrate at a concentration of 1 g/L served as the best nitrogen sources for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyhydroxyalkanoates; Cane molasses; Infrared spectroscopy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100020
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Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells BABT
Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A..
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405
Registros recuperados: 4
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